{"product_id":"handbook-of-cryo-preparation-methods-for-electron-microscopy","title":"Handbook of Cryo-Preparation Methods for Electron Microscopy","description":"\u003cp\u003eWhile new discoveries have led to much dramatic growth in cryo-electron microscopy, researchers will never be able to take full advantage if they lack access to the details that make these techniques understandable and applicable.\u003c\/p\u003e\u003cp\u003eThe \u003cb\u003eHandbook of Cryo-Preparation Methods for Electron Microscopy\u003c\/b\u003e provides researchers with a complete reference that will show them how to equip their labs with the right materials and methods to take full advantage of the latest advances. This pioneering work brings together a group of internationally renowned researchers, some the very inventors of the methods they describe, to share their knowledge and recipes. Taking care to explain the history behind the techniques and to demonstrate their use, this book presents the latest theory, principles, and protocols supplemented by hundreds of illustrations. \u003c\/p\u003e\u003cp\u003eContributions to this handbook – \u003c\/p\u003e\u003cp\u003eÖ Describe cryo-methods aimed at perfect preservation for fine structural analysis \u003c\/p\u003e\u003cp\u003eÖ Teach how to arrest physiological processes by cryo-fixation \u003c\/p\u003e\u003cp\u003eÖ Reveal the secrets for high-resolution snapshots of life by CEMOVIS \u003c\/p\u003e\u003cp\u003eÖ Cover applications of cryo-methods such as electron crystallography, 2D\/3D structure analysis, protein localization, and cryo-electron tomography \u003c\/p\u003e\u003cp\u003eÖ Demonstrate the use of vitreous water as an intermediate step for localization of biological ions and molecules \u003c\/p\u003e\u003cp\u003eÖ Present hybrid methods of freeze-substitution and freeze-drying for immunolabeling and determining molecular geography \u003c\/p\u003e\u003cp\u003eÖ Illustrate freeze-fracture, cryo-ultramicrotomy, and resin embedding \u003c\/p\u003e\u003cp\u003eÖ Highlight the Tokuyasu method and the new rehydration technique for highly efficient immunolabeling \u003c\/p\u003e\u003cp\u003eÖ Include summary tables to compare and obtain appropriate criteria \u003c\/p\u003e\u003cp\u003e\u003cb\u003eIncludes a wealth of immediate reference material for daily use \u003c\/b\u003e \u003c\/p\u003e\u003cp\u003eWith clear, step-by-step recipes and lists of tools, ingredients, and suppliers\u003cb\u003e, \u003c\/b\u003ethis handbookprovides researchers with the knowledge and techniques to adopt the latest cryo-methods to their current research.\u003c\/p\u003e","brand":"Taylor \u0026 Francis Inc","offers":[{"title":"Default Title","offer_id":54255950987608,"sku":"9780849372278","price":341.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0278\/1295\/4195\/files\/9780849372278.jpg?v=1777273263","url":"https:\/\/agendabookshop.com\/products\/handbook-of-cryo-preparation-methods-for-electron-microscopy","provider":"Agenda Bookshop","version":"1.0","type":"link"}