A01=Irwin M. Chaiken
Analytical Affinity Chromatography
Apparent Rate Constant
Author_Irwin M. Chaiken
Biospecific Ligand
Blue Sepharose
Category=PNF
Charles Delisi
Cibacron Blue F3GA
Dissociation Rate Constant
Earle Stellwagen
Elution Volume
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Harold E. Swaisgood
Herbert W. Hethcote
Horse Liver Alcohol Dehydrogenase
IgA Monomer
Isocratic Method
Lactate Dehydrogenase
LDH
Mobile Phase
Monovalent Interaction
Neurohypophysial Hormone
Nonporous Beads
Octopine Dehydrogenase
Plate Height
Reactive Blue
Rodney R. Walters
Sepharose CL-6B
Stagnant Mobile Phase
Typical Elution Profile
uM NADH
Yin-Chang Liu
Zonal Chromatographic
Zonal Elution
μM NADH
Product details
- ISBN 9781315890579
- Weight: 453g
- Dimensions: 178 x 254mm
- Publication Date: 29 Nov 2017
- Publisher: Taylor & Francis Ltd
- Publication City/Country: GB
- Product Form: Hardback
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This volume presents discussions of theoretical and experimental considerations that have led to the analytical affinity chromatography field, as well as current efforts to use this methodology to characterize the interaction mechanisms of biological macromolecules and to establish conditions for employing bioaffinity chromatographic systems as preparative tools. The chapters include a comprehensive discussion of interactive chromatography theory (DeLisi and Hethcote), a review of experimental data obtained for biological macromolecules and the relevant theoretical considerations of affinity chromatography which led to them (Swaisgood and Chaiken), an evaluation of rate processes in affinity chromatography and the potential to determine biologically meaningful chemical rate constants (Walters), and the use of quantitative and molecular considerations to design affinity chromatographic systems (Stellwagen and Liu).
