A01=W. Antoni Kudlicki
Amber Codons
Amber Suppressor tRNA
Author_W. Antoni Kudlicki
Category=PS
Cell Free Expression
Cell Free Expression System
Cell Free Methods
Cell Free Protein Expression
Cell Free Protein Synthesis
Cell Free Protein Synthesis System
Cell Free Reaction
Cell Free Synthesis
Cell Free Systems
Cell Free Translation
Cell Free Translation System
Codon Pair
Coli Cell Free System
Energy Source
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eq_isMigrated=1
eq_isMigrated=2
eq_nobargain
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Fluorescence Cross-correlation Spectroscopy
High Throughput Screening
Nappa
Protein Microarray
Random Peptide Library
Ribosome Display
Single Chain Antibodies
Suppressor tRNA
Translational Pause
Product details
- ISBN 9781587061233
- Weight: 476g
- Dimensions: 152 x 229mm
- Publication Date: 27 Nov 2007
- Publisher: Taylor & Francis Ltd
- Publication City/Country: GB
- Product Form: Hardback
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Following its inception in the 1950s, cell-free protein synthesis made a tremendous impact on the basic life sciences. The use of cell-free systems was key to understanding molecular mechanisms underlying one of the most complicated processes found in nature: protein translation. Since this time, aggressive cutting-edge research and stiff commerical competition have driven the development of a variety of systems with increased productivity, improved protein quality and relatively low production costs. As a result, technology has generated myriad applications that have enabled advances in fields as diverse as systems biology, structural biology, and drug discovery. Cell-Free Protein Expression describes and expands upon many of these applications. The volume has been divided into six main sections. In the first section, many of the most popular sources of cell-free lysates are introduced. The second section focuses on extraordinary advances made in the Escherichia coli-based systems that have enabled reconstitution of the entire translational process, incorporation of post-translational modifications, yield increase, and production of functional membrane proteins. This progress extends the usefulness of cell-free systems into structural biology applications described in the third section and high-content platforms like protein microarrays discussed in the fourth section. The final two sections cover the use of cell-free protein expression technologies in the rational design and directed evolution of proteins within the scientific community.
