advanced RNAi experimental methods
caenorhabditis
Category=PSAK
Category=PSB
Category=PSD
Category=YPMP1
cellular delivery systems
DNA Oligonucleotide
EGFP Express
elegans
eq_bestseller
eq_isMigrated=1
eq_isMigrated=2
eq_nobargain
eq_non-fiction
eq_science
falciparum
function
functional genomics
gene expression analysis
GFP mRNA
GFP Silence
MinElute PCR Purification Kit
molecular biology techniques
NGM Agar Plate
NGM Plate
Nuclease Free Water
Nucleic Acid
plasmodium
Pol Iii
Posttranscriptional Gene Silencing
RISC Complex
RNA Dependent DNA Methylation
RNA Extraction Buffer
RNA Induce Silence Complex
RNA Pol III
RNA Silence
RNAi Reagent
RNase Free Water
RNase III
Short RNA Duplex
siRNA synthesis protocols
studying
T7 RNA Polymerase
transcription
Transfection Efficiency
transgenic model organisms
Tudor Staphylococcal Nuclease
vector
viral
vitro
Vitro Transcription
Product details
- ISBN 9780849321412
- Weight: 703g
- Dimensions: 156 x 234mm
- Publication Date: 27 Aug 2004
- Publisher: Taylor & Francis Inc
- Publication City/Country: US
- Product Form: Hardback
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Maximizing the potential of RNA interference in functional genomics - as well as in the development of therapeutics - continues to be at the forefront of biomedical research. Unlike journal articles, Gene Silencing by RNA Interference: Technology and Application combines essential background to the RNAi field with practical techniques designed by renowned researchers to provide the most diverse and in-depth examination of the subject yet.
This book describes methods and protocols for gene silencing and RNA interference. Each chapter provides necessary background to the subject and then gives detailed methods in easy-to-follow steps, along with troubleshooting hints and tips. Following a general and historical introduction, chapters two through eight focus on technical details of the various methods of siRNA design, chemical and enzymatic production, plasmid- and virus-mediated intracellular expression, and other RNAi tools. Chapter nine discusses the exogenous delivery of siRNAs into cells, and the final chapters of the book detail the application of RNAi to dissect gene function in a number of biological systems, including cell-free systems, cultured cells, and whole organisms.
From the design and production of RNAi tools to their applications, Gene Silencing by RNA Interference: Technology and Application is the first to present the entire spectrum of activity, design, production, and delivery of RNAi reagents, providing a welcome guide for both academic and commercial use.
