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Handbook of Detection of Enzymes on Electrophoretic Gels

Gennady P. Manchenko
€78.99
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Achromatic Bands
Aptamer Beacons
Author_Gennady P. Manchenko
bioautography methods
buffer systems in gels
Category=PSAK
Category=PSB
Category=PSD
chromogenic detection
Diazonium Salt
DNA Marker
DNA Substrate
Electrophoretic Gels
Electrophorized Gel
enzyme isoform characterization
enzymes purification
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Fast Blue RR Salt
gel electrophoresis
Glucose-6 Phosphate Dehydrogenase
isozymes
molecular genetics
NADPH Dependent Reaction
Polyribonucleotide Nucleotidyltransferase
protein electrophoresis
Protein Set
Reduce Tetrazolium Salts
Tetrazolium Salts
zymogram analysis

Product details

  • ISBN 9780367454616
  • Weight: 1050g
  • Dimensions: 210 x 280mm
  • Publication Date: 02 Dec 2019
  • Publisher: Taylor & Francis Ltd
  • Publication City/Country: GB
  • Product Form: Paperback
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Description

Still widely used as gene markers, isozymes detected by zymogram techniques have proven valuable in a range of other biological applications over the last few years. Along with these new applications, many new techniques have also emerged. Yet more than eight years since the Handbook of Detection of Enzymes on Electrophoretic Gels was first published, it remains the only book completely devoted to zymogram methods. The time has come to bring its contents up to date.

New in the Second Edition:

  • An overview of new applications of enzyme electrophoresis and zymogram techniques
  • Zymogram techniques for approximately 100 enzymes not included in the first edition, bringing the total to more than 900 methods for detecting more than 400 different enzymes
  • Information on subunit structure included within the enzyme sheets to facilitate interpretation of isozyme patterns detected on zymograms
  • An appendix containing information on the buffer systems most commonly used for enzyme electrophoresis in starch, cellulose acetate, and polyacrylamide gels.

    The second edition of this bestselling handbook makes significant additions to the set of reliable gene markers suitable for electrophoretic analysis. It also strengthens the value of enzyme electrophoresis as a powerful tool proven successful in solving a variety of problems encountered across many biological areas, including the post-genome biology.
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    About Gennady P. Manchenko
    Gennady P. Manchenko, Ph.D., is senior researcher at the Laboratory of Genetics at the Institute of Marine Biology of the Russian Academy of Sciences at Vladivostok.

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