In Situ Hybridization in Electron Microscopy
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A01=Annie Cavalier
A01=Gerard Morel
A01=Lynda Williams
acid
acrylic
antigenic
Antigenic Probe
Asymmetric PCR
Author_Annie Cavalier
Author_Gerard Morel
Author_Lynda Williams
Autoradiography Emulsion
buffer
Category=PDN
Category=PH
Category=PSD
Category=PSF
cDNA Probes
Cell Monolayers
Cryogenic Agents
Cryoprotective Agents
DNA Probe
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eq_isMigrated=1
eq_isMigrated=2
eq_nobargain
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ERL
frozen
functional dynamism
gene localization methods
Hybridization Buffer
Immuno Cytochemistry
Lowicryl K4
LR White Resin
microscopy sample preparation
molecular cytogenetics
nucleic
nucleic acid detection
Oligonucleotide Probes
PCR Protocol
probes
Radioactive Hybrids
Radioactive Probes
resin
resin embedding protocols
Sample Preparation
scanning tunneling microscopy
Semithin Sections
Synthetic DNA
target
Target Nucleic Acid
tissue
tissue section analysis
ultrastructural gene visualization techniques
ultrastructural visualization
Ultrathin Sections
Unstable Hybrids
Vibratome Sections
Vitro Transcription
Product details
- ISBN 9780849300448
- Weight: 1150g
- Dimensions: 178 x 254mm
- Publication Date: 30 Jan 2001
- Publisher: Taylor & Francis Inc
- Publication City/Country: US
- Product Form: Hardback
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In situ hybridization is a technique that allows for the visualization of specific DNA and RNA sequences in individual cells, and is an especially important method for studying nucleic acids in heterogeneous cell populations. in situ Hybridization in Electron Microscopy reviews the three main methods developed for the ultrastructural visualization of genes:
° hybridization on ultrathin sections of tissue embedded in hydrophilic resin (post-embedding method)
° hybridization prior to embedding (pre-embedding)
° hybridization on ultrathin sections of frozen tissue (frozen tissue method).
For each technique, the different stages are described in detail: the preparation of tissue, pretreatment, hybridization, and visualization of the hybridization products.
The book combines theory and practice, starting with the basic principles, then breaking down the experimental process into successive steps illustrated by numerous diagrams, detailed protocols, and tables. This is all done in a format that uses parallel columns to convey useful comments next to the theory and practical details alongside each stage of the protocol. Additionally, the summary tables provide the criteria for choosing the probe type and technique, and a detailed index aids in the search for information. in situ Hybridization In Electron Microscopy is an essential companion for applying these methods at the electron microscopic level.
Morel, Gerard | Cavalier, Annie | Williams, Lynda
