Isolation and Purification of Proteins

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adsorption
Adsorption Buffer
Affinity Chromatography
Affinity Ligands
Affinity Precipitation
Amersham Pharmacia Biotech
Average Specific Cake Resistance
Axial Dispersion
Backward Extraction
bed
Bovine IgG
Category=PS
chromatography
dekker
eq_bestseller
eq_isMigrated=1
eq_isMigrated=2
eq_nobargain
eq_non-fiction
eq_science
Eudragit S-100
expanded
Expanded Bed
Expanded Bed Adsorption
Fast Flow Sepharose
Forward Extraction
Grade Efficiency
Hic
hydrophobic
interaction
marcel
Mobile Phase
Moisture Content
Packed Bed
Plate Height
Procion Red H E7B
Purification Factor
recombinant
Reversed Micellar Phase
Reversed Micelles
Solid Diffusion
target

Product details

  • ISBN 9780367395490
  • Weight: 1256g
  • Dimensions: 152 x 229mm
  • Publication Date: 19 Jun 2019
  • Publisher: Taylor & Francis Ltd
  • Publication City/Country: GB
  • Product Form: Paperback
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This publication details the isolation of proteins from biological materials, techniques for solid-liquid separation, concentration, crystallization, chromatography, scale-up, process monitoring, product formulation, and regulatory and commercial considerations in protein production. The authors discuss the release of protein from a biological host, selectivity in affinity chromatography, precipitation of proteins (both non-specific and specific), extraction for rapid protein isolation, adsorption as an initial step for the capture of proteins, scale-up and commercial production of recombinant proteins, and process monitoring in downstream processing.
Rajni Hatti-Kaul, Bo Mattiasson