PCR Protocols in Molecular Toxicology

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?l Genomic DNA
A01=John P. Vanden Heuvel
advanced PCR applications in toxicology research
Analyze PCR Product
Author_John P. Vanden Heuvel
Category=PSB
Category=PSD
cDNA Reaction
cellular response mechanisms
cloning strategies
Competitive Rt PCR
DD
enzyme polymorphism detection
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gene expression analysis
GSP
GST Gene Family
high-throughput genotyping methods
HSV Tk
Insert DNA
MMLV Reverse Transcriptase
modern molecular toxicology
molecular biology techniques
Multiplex PCR
Oligo dT Adapter Primer
PCR Mix
PCR Product
PCR Protocol
PCR RFLP Genotyping
PCR Sample
PCR Tube
PCR-based polymorphism assays
Plasmid DNA
Protein DNA Interaction
quantitative reverse transcriptase PCR protocols
SM Buffer
Target PCR Product
Thermostable DNA Polymerase
Thymidine Kinase
toxicogenomics
Vitro Transcription
Μl Genomic DNA

Product details

  • ISBN 9780849333446
  • Weight: 376g
  • Dimensions: 156 x 234mm
  • Publication Date: 24 Sep 1997
  • Publisher: Taylor & Francis Inc
  • Publication City/Country: US
  • Product Form: Paperback
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Molecular toxicology is an emerging discipline that utilizes molecular and cell biology to understand how drugs and chemicals result in their unwanted effects. PCR Protocols in Molecular Toxicology is a practical guide to the use of polymerase chain reaction (PCR) to help examine, on a molecular and cellular level, how toxic responses are manifested. It offers a basic understanding of PCR and its optimization, as well as describing specific, high-impact areas of molecular toxicology and recent advances. The following techniques are described in detail:

  • Quantitative reverse transcriptase PCR and methods to examine gene expression
  • Differential display cloning
  • Cloning and library screening by PCR
  • Genotype and polymorphism analysis of drug and toxicant metabolizing enzymes
  • Basic, non-PCR based molecular biology methods

    PCR Protocols in Molecular Toxicology will aid both novices and experienced PCR practitioners in using PCR to its fullest potential.
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