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A01=Gerard Morel
A01=Mireille Raccurt
acid
advanced microscopy techniques
Al De
amplification
Anti-sense Primer
Aqueous Uranyl Acetate
Ar Af
Author_Gerard Morel
Author_Mireille Raccurt
CaCl2 Buffer
Category=PDND
Category=PSAK
Direct PCR
DNA Matrix
DNA Polymerase
DNA Polymerase Act
DNA Target Sequence
eq_bestseller
eq_isMigrated=1
eq_isMigrated=2
eq_nobargain
eq_non-fiction
eq_science
Fi Xa
gene expression detection
hybridization
Hybridization Temperature
in situ PCR protocol optimization
medium
molecular pathology
NaCl Buffer
Ne Ga
Nonspecific Hybridization
nucleic
PCR. DNA
product
reaction
reverse
RNA Target Sequence
RNase Free Conditions
Rt PCR Method
Rt PCR Step
Taq DNA Polymerase
temperature
tissue sample analysis
transcription
Tris HCl Buffer
tumor biomarker identification
viral genome localization
Xi Ge
Product details
- ISBN 9780849300417
- Weight: 1014g
- Dimensions: 178 x 254mm
- Publication Date: 27 Sep 2002
- Publisher: Taylor & Francis Inc
- Publication City/Country: US
- Product Form: Hardback
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Although the polymerase chain reaction has revolutionized genetic analysis by amplifying rare nucleic acid sequences, the in situ application is the only method that allows the localization of amplified signal within tissue structure. The applications of in situ polymerase chain reaction have greatly enhanced the field of investigation in many disciplines, including viral infections, gene modification, tumor diagnosis, gene therapy, and cellular distribution of rare mRNA copies.
PCR/RT-PCR in situ: Light and Electron Microscopy covers methods of in situ polymerase chain reaction (PCR) and reverse transcription PCR (RT-PCR), two new approaches in visualizing very low amounts of DNA and RNA in tissues and cell cultures at the light and electron microscopy levels. Written by experts in this field, the book provides theoretical consideration, as well as practical approaches to in situ PCR. The authors provide detailed protocols for each step, including the preparation of tissue samples, the rationale for the design of primers and revelation. They also emphasize the need for appropriate controls to meet the requirements of in situ PCR and RT-PCR specificity. Organized in a user-friendly two-column format, this book will provide you with tools necessary to perform and optimize these sensitive and powerful techniques in your research protocols.
Gérard Morel, Ph.D., D.Sc., is a research director at the National Center of Scientific Research (CNRS), at University Claude Bernard-Lyon 1, Villeurbanne, France. Mireille Raccurt, Ph.D., is an engineer in biology in a laboratory of the CNRS (Molecular Physiology), at Claude Bernard-Lyon 1 University, Villeurbanne, France.
