Practical Fluorescence Spectroscopy

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A01=Ignacy Gryczynski
A01=Zygmunt (Karol) Gryczynski
advanced fluorescence experiment protocols
advanced time-resolved fluorescence
Anisotropy Decays
Author_Ignacy Gryczynski
Author_Zygmunt (Karol) Gryczynski
biomedical fluorescence analysis
biophysical measurement techniques
Category=PHVN
Category=PNFS
Category=PSB
Corrected Fluorescence Spectra
Emission Anisotropies
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experimental photophysics
Fluorescence Anisotropy
Fluorescence Anisotropy Decay
Fluorescence Cuvette
Fluorescence Intensity Decay
Fluorescence Lifetime
Fluorescence Spectra
fluorescence spectroscopy
Forster Resonance Energy Transfer (FRET)
handle artifacts
HBSS Buffer
Intensity Decay
KI Concentration
laboratory data interpretation
Light Quenching
Measure Fluorescence Spectra
Measured Emission Spectra
molecular probe dynamics
PBS Buffer
Plastic Cuvette
practical guidance
Pva Absorption
PVA Film
Pva Solution
quantitative spectroscopy methods
R6G
R6G Solution
steady-state fluorescence
steady-state phenomena
Stern Volmer Plots
time-resolved phenomena
Ultra-Fast Pulsed Lasers
UV Illuminator
UV Spectral Range

Product details

  • ISBN 9781439821695
  • Weight: 1630g
  • Dimensions: 178 x 254mm
  • Publication Date: 11 Dec 2019
  • Publisher: Taylor & Francis Inc
  • Publication City/Country: US
  • Product Form: Hardback
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Presenting a detailed, hands-on approach to fluorescence spectroscopy, this book describes experiments that cover basic spectroscopy and advanced aspects of fluorescence spectroscopy. It emphasizes practical guidance, providing background on fundamental concepts as well as guidance on how to handle artifacts, avoid common errors, and interpret data. Nearly 150 experiments from biophysics, biochemistry, and the biomedical sciences demonstrate how methods are applied in practical applications. The result is a hands-on guide to the most important aspects of fluorescence spectroscopy, from steady-state fluorescence to advanced time-resolved fluorescence.

  • Provides a complete overview of nearly 150 experiments using fluorescence spectroscopy, from basic to advanced applications
  • Presents laboratory methods using a variety of instrumental setups with detailed discussion of data analysis and interpretations
  • Covers steady-state phenomena, time-resolved phenomena, and advanced methods
  • Spans biophysical, biochemical, and biomedical applications
  • Describes related concepts, theory, and mathematical background as well as commercially available instruments used for measurements

Zygmunt (Karol) Gryczynski is a professor of molecular biology and immunology and the director of the Center for Commercialization of Fluorescence Technologies (CCFT) the University of North Texas Health Center.

Ignacy Gryczynski is a professor of cell biology and genetics and the director of the Microscopy Core Facility at the University of North Texas Health Center.

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