Principles and Reactions of Protein Extraction, Purification, and Characterization

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A01=Hafiz Ahmed
A01=Hafiz Ahmed PhD
Acrylamide Stock Solution
adjuvant
advanced protein separation strategies
Ammonium Persulfate
Amplex Red Reagent
Anion Exchange Chromatography
antibody production methods
assay
Author_Hafiz Ahmed
Author_Hafiz Ahmed PhD
Biotin Hydrazide
Bolton Hunter Reagent
buffer
Category=PSB
Category=PSD
complete
Desalting Column
Destaining Solution
determine
electrophoresis applications
Eluting Buffer
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eq_isMigrated=1
eq_isMigrated=2
eq_nobargain
eq_non-fiction
eq_science
Equilibrating Buffer
Fast Red TR
freunds
Gel Buffer
Gel Cassette
Gel Filtration Chromatography
glycoprotein analysis
Hat Medium
Homobifunctional Cross-Linkers
IEF
IgG Solution
immunoassay techniques
lowry
Lowry Assay
Mass Spectrometry
Methionine Free Medium
NMR
Non-denaturing Page
oligosaccharide profiling
Primary Antibody
Protein Characterization
Protein Extraction
Protein Purification
protein quantification
Recombinant Proteins
Stacking Gel
Stacking Gel Buffer
Staining Solution
standard
triton
x-114

Product details

  • ISBN 9780849320347
  • Weight: 750g
  • Dimensions: 156 x 234mm
  • Publication Date: 15 Sep 2004
  • Publisher: Taylor & Francis Inc
  • Publication City/Country: US
  • Product Form: Paperback
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Principles and Reactions of Protein Extraction, Purification, and Characterization provides the mechanisms and experimental procedures for classic to cutting-edge techniques used in protein extraction, purification, and characterization. The author presents the principles and reactions behind each procedure and uses tables to compare the different methods. The book also discusses the development of antibodies and immunochemical techniques as tools for characterizing proteins and modified proteins such as glycoproteins. Helpful illustrations, diagrams, and tables effectively transform theoretical concepts into practical knowledge. Along with methodical working procedures for most techniques, the book also offers useful advice on which technique to use and when to apply a particular method. Presenting the advantages and disadvantages of the various protein techniques, Principles and Reactions of Protein Extraction, Purification, and Characterization enables students and researchers to master the mechanisms behind the protocols and choose the best method for their purposes.

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